The Tenderloins comedic sketches have accumulated millions of views on YouTube, MySpace, Metacafe, and other websites. In 2007, the troupe won the $100,000 grand prize in the NBC It’s Your Show competition for the sketch “Time Thugs”.

Following the troupe's online successes, the four were signed to develop a scripted comedy pilot for Spike TV based on their own lives. The pilot was shot on location in 2008 on Staten Island, as well as at other locations around New York City. Spike TV ultimately passed on the series. A second pilot starring The Tenderloins was officially selected for the 2009 New York Television Festival

On April 12, 2011, truTV announced a new series to star the Tenderloins. Impractical Jokers premiered on December 15, 2011, executive-produced by the troupe themselves. Season 1 consisted of 17 half-hour episodes. The first season was watched by over 32 million viewers.

The show's 28-episode second season premiered on December 13, 2012,[3] and its 31-episode third season premiered on January 2, 2014.[4] On April 21, 2014, truTV announced that the series would be renewed for a 26-episode fourth season.[5] With the fourth season renewal, a 6-episode spinoff series, Jokers Wild! was also announced.[5] The show was renewed for a fifth season which first began on February 2016. On July 22, 2016, a sixth season was announced.

On February 2017, Murr tweeted that he and the group had recorded the first episode of an upcoming limited-run show for SiriusXM Radio. On August 17, 2017, The Tenderloins Radio Show made its debut on SiriusXM Radio channel 94 (Comedy Greats). In the hour-long broadcast, the comedy troupe pranks friends and acquaintances, takes calls, answers emails from fans, and does sketches in a segment called "Tenderloins Theatre".

"What Say You?", a podcast hosted by Sal and Q and released fairly regularly, was named Best New Show at the 2013 Stitcher Awards.[6] The increased popularity of "What Say You" sparked a friendly competition among the friends, spurring Joe and Murr to release their own Tenderloins podcast without the other two members. In 2015, "What Say You?" was nominated for the Comedy, Entertainment, and Best Produced Podcast Awards at the 10th Annual Podcast Awards.[7] Sal and Q have stated during their podcast that it their own side project, not a replacement of The Tenderloins podcast. The group explained that it was difficult to coordinate the schedules of all four members outside of work making it challenging to produce their troupe's official podcast with any regularity.